Scientists Have Successfully Built a Custom, Synthetic Chromosome from Scratch

[Buster's Uncle]

Scientists Have Successfully Built a Custom, Synthetic Chromosome from Scratch
The Atlantic Wire
By Polly Mosendz  March 28, 2014 4:21 PM



The first designer chromosome is here. After seven years, a team of international scientists has created the first artificial eukaryote chromosome from scratch.   

Eukaryotes are complex cell organisms, like humans and plants, “in which the genetic material is organized into a membrane-bound nucleus.” The chromosome, was incorporated into brewer’s yeast, was built using a computer redesign of the structure. Scientists created the “thread-like structure piece by piece in the laboratory.”  Each gene was removed and replaced with fresh DNA, until an entirely new chromosome was constructed.

While bacteria and virus chromosomes have been created in the past, an artificial eukaryote chromosome is a major scientific development. The recreated Saccharomyces cerevisiae chromosome is of special significance because it determines how well the organisms mate. The chromosome was transplanted into a healthy organism, where it worked normally. In fact, it worked better than normally. Dr. Jef Boeke, head of the Institute of Systems Genetics at NYU, found that “they behave almost identically to wild yeast cells, only they now possess new capabilities and can do things that wild yeast cannot.”

Among biologists, the accomplishment has been compared to the first Mount Everest climb. Scientist Patrick Yizhi Cai believes “This is a major step forward. It’s the first synthetic eukaryote. This really demonstrates that we can do rational design on the chromosome scale. Ten years from now we’ll be able to synthesis genomes on a day-to-day basis.

The study of yeast chromosomes opens the door for the “biotechnology industry to produce everything from alcohol […] to biofuels and specialty chemicals to nutrients.” Yeast and human cells have a similar genetic material packaging, both using a nucleus for DNA containment. Because of this similarity, Dr Boeke notes that yeast “serves as a better model for how human cells work in health and disease”. 

Though scientists are enthused, we don’t have to worry about a GATTACA world any time soon. Scientists believe it will take four years to create the 16 complete yeast chromosomes, and that's still a long way building our humans from scratch.


http://news.yahoo.com/scientists-successfully-built-custom-synthetic-chromosome-scratch-202128029.html

[JarlWolf]

This is a step towards progress.

[Buster's Uncle]

Scientists just took a major step toward making life from scratch
For the first time, researchers have synthesized a eukaryotic chromosome in a lab
By Arielle Duhaime-Ross on March 27, 2014 02:00 pm






Synthetic biology has come a long way in recent years. In the last two decades alone, scientists have been able to go from synthesizing the genome of a relatively small virus, Hepatitis C, to creating what researchers refer to as the "first synthetic cell" from a unicellular organism. Yet until recently, researchers had been incapable of constructing one of the most emblematic symbols of our own genetic makeup: the eukaryotic chromosome. Now, a team of scientists has announced that the age of the synthetic chromosome is upon us, as a study published in Science today reveals how the group was able to construct a yeast chromosome from scratch — an experiment that allowed the team to make fully functional "designer yeast."


Yeast cells with "unusual properties."


Eukaryotic chromosomes belong to eukaryotes — organisms such as animals, plants, and yeast whose cells contain a membrane-bound nucleus. Although scientists have previously been able to construct viral DNA and bacterial DNA, the synthesis of a eukaryotic chromosome had not been achieved. So, when the scientists decided to construct a chromosome from scratch, they knew they had to plan it out carefully. "We didn't make a carbon copy of an existing chromosome," says Jef Boeke, a molecular biologist at New York University and co-author of the study, "but an extensively modified version designed on a computer, using a set of principles that were predicted to make happy, healthy yeast."

This careful planning is what allowed the researchers, along with 60 undergraduate students, to painstakingly string chunks of DNA together and insert them into living yeast cells. It's also what allowed them to introduce over 500 changes to the chromosome's native sequence — a process that yielded yeast cells endowed with what Boeke referred to as "unusual properties."


Rearranging the chromosome "on demand."


One of the most significant changes they introduced was the addition of a gene called "Cre". This gene is atypical because it produces a protein, also called "cre," that can scramble the synthetic chromosome's sequence when it comes in contact with estrogen — the human sex hormone. This technique is called "the scrambling approach," and it allows the researchers to rearrange the structure of the designer chromosome "on demand" within the living yeast cells, just by adding various concentrations of estrogen to the growth medium, Boeke explains. "So, just like the shuffling of a set of cards, you can delete or duplicate any subset of genes and generate a whole new set of cards — or a whole new genetic sequence."

The researchers hope to use the scrambling method to come up with yeast that can tolerate a wider range of environmental conditions, and that can carry out fermentation more efficiently. If they can do that, the applications will be countless, because these microorganisms do a lot more than help us make beer and bread. "I think we will see all kinds of biosynthetic products made in bacteria and yeast over the next 10 years," Boeke says. This advancement will make the production of things like antimalarial drugs and diesel fuel-like compounds a lot more cost-effective, he says. "Pretty much anything made in yeast could benefit from this scrambling approach."


"We will be able to do a lot more when we can control all of its genes."


But wholly engineered designer yeast isn't on its way just yet. There is a lot more work to do before researchers can truly explore the treasure trove of applications that this technique will engender, because yeast has more than one chromosome. In fact, it has 16. "It's unlikely that we will revolutionize an industry by rearranging a single chromosome," says Boeke. But the scientists might be able to revolutionize a number of industries if they can synthesize the whole set. "Ultimately we want to do this with all 16," Boeke says, which should take the researchers another two to three years. "That's when it will become really interesting and powerful, because we will be able to do a lot more when we can control all of its genes."

Boeke knows some people might question the wisdom of "controlling genes" in this manner, but he doesn't take those criticisms very seriously. "Unless they subsist exclusively on fruits, nuts and fish, there is about a 100 percent chance they are enjoying the meddling done by our genetically oriented forebears who did selective breeding." And in any case, he says, whenever the designer chromosome gets too scrambled, "it deletes itself out, self-destructs and the yeast dies," so the dangers of these types of interventions are minimal.


http://www.theverge.com/2014/3/27/5553044/first-functional-eukaryotic-chromosome